HeLa Nuclear Extract (DTT-free)
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Catalog number
7947-1
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Price
Please ask
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Size
1 mg
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Description
For western blotting and HDAC assay positive controls.. Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
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Product Highlights
• Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, and 1 mM PMSF. •Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay
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Storage Temp
-70°C
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Shipping
dry ice
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
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Additional description
Pure extracts used in broths or as control for cell culture and Western Blot.
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