HeLa Nuclear Extract
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Catalog number1641-01
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PricePlease ask
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Size100 μg
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DescriptionFor western blotting and HDAC assay positice controls.. Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
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Product Highlights• Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF. •Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay
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Storage Temp-70°C
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Shippingdry ice
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Shelf Life12 months
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NotesFor research use only. Not to be used for human or animal treatment or consumption.
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Additional descriptionPure extracts used in broths or as control for cell culture and Western Blot.
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Gene target
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Gene symbolDENND2B
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Short nameHeLa Extract
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Techniqueextract, extracts
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Alternative nameHeLa Nuclear Extract
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Alternative techniqueextrats
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Gene info
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Identity
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Gene
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Long gene nameDENN domain containing 2B
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Synonyms gene
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Synonyms gene name
- suppression of tumorigenicity 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-07-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DENN domain containing
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VEGA ID