HeLa Nuclear Extract

  • Catalog number
    1641-01
  • Price
    Please ask
  • Size
    100 μg
  • Description
    For western blotting and HDAC assay positice controls.. Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
  • Product Highlights
    • Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF. •Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay
  • Storage Temp
    -70°C
  • Shipping
    dry ice
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Additional description
    Pure extracts used in broths or as control for cell culture and Western Blot.
  • Gene target
    HeLa  
  • Gene symbol
    DENND2B
  • Short name
    HeLa Extract
  • Technique
    extract, extracts
  • Alternative name
    HeLa Nuclear Extract
  • Alternative technique
    extrats
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