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Synonyms
Ck2a1
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Alternative_names
Ck2a1
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Description
Phosphorylates acidic proteins such as casein
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Recombinant
Yes
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Source
Baculovirus (Sf9 insect cells)
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
107 nmol phosphate incorporated into casein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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Molecular Weight
70.0 kDa
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Storage Temp
-80°C
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Shipping
dry ice
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Shelf Life
12 months
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Concentration
5µg/50 µl
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Appearance
Liquid
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Physical form description
Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
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Background Information
CK2 α (also termed casein kinase II α) is a serine-threonine protein kinase whose targets include many critical regulators of cellular growth. It is highly expressed in a lympho pro-liferative disease of cattle and in many human cancers. Overexpression of the CK2 α catalytic subunit in lymphocytes of transgenic mice leads to T cell lymphoma (1). The highest CK2 activity is found in mouse testicles and brain, followed by spleen, liver, lung, kidney and heart (2). The activity values were directly correlated with the protein expression level of the CK2α (catalytic subunit). The α subunit is only detected in brain and testicles. By contrast, Northern blot analyses of the CK2 α mRNA shows the strongest signals to be present in brain, liver, heart and lung. In kidney, spleen and testicles mRNAs is only weakly detectable. ICBP90, a transcription factor exhibiting antiapoptotic property, has several putative CK2 phosphorylation sites. ICBP90 is more efficiently phosphorylated by the free CK2 α subunit than by the heterotetrameric CK2 (α, β) (3). Thus, CK2 α is an important regulator of the transcriptional activity of ICBP90 and therefore of the antiapoptotic properties of ICBP90.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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