This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti- Estrogen Sulfotransferase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
EST, EST-1 , MGC34459 , STE , SULT1E1
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. Detects 31 kDa Estrogen Sulfotransferase (EST) of human and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Synthetic peptide surrounding amino acid 195 of human Estrogen Sulfotransferase (EST)
How to handle this antibody:
The antibody solution should be gently mixed before use.
Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3’-phosphate-5’-phosphosulfate. Sulfurylated steroids do not bind to the estrogen receptor with high affinity and, therefore, are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor-active estrogens.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.