Mutant Isocitrate Dehydrogenase (Mutant IDH) Activity Assay Kit (Colorimetric)

• Catalog number
  K985-100
• Price
  Please ask
• Size
  100 assays

• Description
  The first and only in vitro assay with a simple, enzyme-based, sensitive, non-radiometric protocol for the detection of Mutant IDH.
• Summary
  • Detection method- Absorbance (340 nm) • Species reactivity- Mammalian • Application- This assay has a detection sensitivity as low as 2 mU.
• Detection Method
  Absorbance (OD 340 nm)
• Species Reactivity
  Mammalian
• Applications
  This assay has a detection sensitivity as low as 2 mU.
• Sample Type
  Cell and tissue culture lysates and purified enzyme preparations.
• Features Benefits
  • Simple procedure • Fast and convenient •
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) is an enzyme that catalyzes the decarboxylation of Isocitrate producing α-Ketoglutarate and CO2. Mutations in both isoforms of IDH (IDH1 and IDH2) are commonly found in human cancers. The Mutant Isocitrate Dehydrogenase (Mutant IDH) causes a “gain-of-function”, which reduces its affinity for isocitrate and favors the conversion of α-ketoglutarate to D-2-Hydroxyglutarate. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. D2HG functions as an “oncometabolite” promoting cellular transformation. Recent studies show that increased Mutant IDH activity is associated with various cancers; therefore, detection of Mutant IDH activity is important for diagnosis and developing therapeutic strategies (e.g. Mutant IDH inhibitors). BioVision’s Mutant IDH assay kit provides a quick and simple assay to monitor the Mutant IDH activity in biological samples. In the assay, Mutant IDH oxidizes NADPH into an NADP+, which decreases the absorbance at 340 nm. The assay is simple, sensitive, and can detect Mutant Isocitrate Dehydrogenase activity lower than 2 mU/ml in a variety of cancer samples
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Mutant   Isocitrate   Dehydrogenase   Mutant   IDH   Activity   Kit  
• Short name
  Mutant Isocitrate Dehydrogenase (Mutant IDH) Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Mutant Isocitrate Dehydrogenase (Mutant IDH) Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products