Thrombopoietin Recombinant Protein
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Catalog number
40-285
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
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Other name of the recombinant
ML, TPO, MGDF, MKCSF, MPLLG, THCYT1, Thrombopoietin, C-mpl ligand, ML
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Recombinant in
E. coli
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SEQUENCE
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
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Activity
The ED50 was determined by the dose - dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg.
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Keep at
The lyophilized Thrombopoietin recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Thrombopoietin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Thrombopoietin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
4507493
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Accession nr
NP_000451.1
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
7066
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Gene name
thrombopoietin
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Source
Homo sapiens
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Uniprot
P40225
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
THPO
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Short name
Thrombopoietin Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Thrombopoietin Rec. Protein
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
thrombopoietin
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Synonyms gene
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Synonyms
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Synonyms name
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Locus
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Discovery year
1994-11-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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