ADP Colorimetric Assay Kit II
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Catalog number
K356-100
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Price
Please ask
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Size
100 assays
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Description
Highly Stable, HTS
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Summary
• Detection method - Absorbance (450 nm) • Application - The kit can detect less than 20 μM of ADP in samples.
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Sample Type
• Animal tissues - Liver, Muscle, Heart etc. • Cell culture - Adherent or suspension cells
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Features Benefits
• Simple procedure; takes ~ 20 minutes • Convenient and sensitive • Kit contains all necessary reagents for accurate measurement of ADP levels
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. BioVision ADP Assay kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP in the presence of ADP Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. ADP Assay Kit II is simple, fast and high-throughput ready. It can detect less than 20 μM of ADP in samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbol
WDTC1, ADPGK
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Short name
ADP Colorimetric Assay Kit II
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
ADP colorimetry test reagent II
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Product images
Product files
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