Human Recombinant UCHL5

• Catalog number
  6359-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant UCHL5
• Alternative_names
  Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH37
• Description
  A deubiquitinating enzyme
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: >500 pmole/min/µg. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.
• Molecular Weight
  39.7 kDa (349 aa, 1-329 aa + NT His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 5 mM DTT, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA and 30% glycerol.
• Background Information
  UCHL5 is a member of the peptidase C12 family. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and is the deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UCHL5  
• Gene symbol
  UCHL5
• Short name
  Recombinant UCHL5
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. ubiquitin carboxyl-terminal hydrolase L5
• Alternative technique
  rec
• Alternative to gene target
  ubiquitin carboxyl-terminal hydrolase L5, UCHL5 and IDBG-105454 and ENSG00000116750 and 51377, proteasome binding, nuclei, Uchl5 and IDBG-197081 and ENSMUSG00000018189 and 56207, UCHL5 and IDBG-629660 and ENSBTAG00000013620 and 282110

Gene info

• Identity
  HGNC:19678
• Gene
  UCHL5
• Long gene name
  ubiquitin C-terminal hydrolase L5
• Synonyms gene name
  • ubiquitin carboxyl-terminal hydrolase L5
• Synonyms
  • UCH37
  • CGI-70
  • INO80R
• Synonyms name
  • INO80 complex subunit R
• Locus
  1q31.2
• Discovery year
  2002-11-11
• Entrez gene record
  51377
• Pubmed identfication
  • 10810093
  • 16027725
• RefSeq identity
  • NM_015984
• Classification
  • Ubiquitin C-terminal hydrolases
  • INO80 complex
• VEGA ID
  OTTHUMG00000035561

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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