Virus Nipah (NIV) Reactif III et IV- RT PCR
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Catalog numberNIV FR-0170-02
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PricePlease ask
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Size25tests / kit
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DescriptionDosage PCR en temps réel pour la détection du virus Nipah. c'est une kit prêt à utiliser. cet kit contient un contrôle négatif, un contrôle positif, un mélange, amorce et des sondes. Ce test peut être utilisé immédiatement après l'arrivée du kit. C'est un kit hautement spécifique pour le virus Nipah.
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ApplicationDiagnostic de la contamination par le virus Nipah
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Utilistationdétection du virus Nipah dans le sérum, le plasma, le tissu animal infecté ou la sécrétion en utilisant des systèmes de PCR en temps réel.
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NIVL'infection par le virus Nipah (NiV) est une zoonose virale causée par le virus Nipah du genre Henipavirus chez les animaux et les humains.
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Maladiesencéphalite, maladies respiratoires.
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Hôte du viruschauves-souris frugivores de la famille des Ptéropodidés, qui appartient au genre Pteropus.
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Zone de déclenchementInde, Chine, Bangladesh, Thaïlande…
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Contenu du kitNiV Super Mix, 480μl Mélange d'enzymes RT-PCR, 28μl Eau de qualité moléculaire, 400μl Contrôle interne, 30μl Contrôle positif de NiV, 30μl
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Acide nucléiqueARN
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StockageTous les réactifs doivent être conservés à -20 ° C. Le stockage à + 4 ° C n'est pas recommandé.
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Durée de conservationTous les réactifs peuvent être utilisés jusqu'à la date d'expiration indiquée sur l'étiquette du kit
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PrecautionsIl faut éviter de décongeler et de congeler plusieurs fois (> 3 fois), car cela pourrait réduire la sensibilité du test. Refroidir tous les réactifs pendant les étapes de travail. Super Mix doit être stocké dans l'obscurité.
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InstrumetsPE5700, MJ-Opticon et autres systèmes monochromes et ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gène 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 et autres systèmes multicolores
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbolCPLX3, MGAT3, MGST3, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3, IGHVIII-67-4, IGHVIII-76-1
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Short nameVirus Nipah (NIV) Reactif III et IV- RT PCR
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TechniqueRT PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. RT PCR is reverse transcription polymerase chain reaction to quantitate mRNA messenger RNA that after cDNA transcription. NiV Gen uses this as a fast way to measure gene expression.
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SpeciesVirus, Viruses
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Alternative nameVirus Nipah (NIV) Reactif III et IV- RT PCR test kit
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Alternative techniquedna-amplification
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Gene info
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Identity
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Gene
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Long gene namecomplexin 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
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Synonyms gene name
- mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit alpha 3
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
- prolyl 4-hydroxylase, alpha polypeptide III
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 5
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Synonyms gene name
- caspase 5, apoptosis-related cysteine protease
- caspase 5, apoptosis-related cysteine peptidase
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Synonyms
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Locus
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Discovery year1996-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-82 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-82
- immunoglobulin heavy variable (III)-82 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrially encoded conserved sequence block III
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Synonyms gene
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Synonyms gene name
- conserved sequence block III
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Synonyms
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Locus
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Discovery year1989-10-11
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Pubmed identfication
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Classification
- Mitochondrially encoded regions
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-67-4 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-67-4
- immunoglobulin heavy variable (III)-67-4 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-76-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-76-1
- immunoglobulin heavy variable (III)-76-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
- E05.393.620.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data