Virus Nipah (NIV) Reactivo III y IV-RT PCR

• Catalog number
  NIV ES-0170-02
• Price
  Please ask
• Size
  25tests / kit

• Descripción
  Análisis de PCR en tiempo real para la detección del virus Nipah. es un kit listo para usar. este kit contiene un control negativo, un control positivo, una mezcla, un cebador y sondas. Esta prueba se puede usar inmediatamente después de la llegada del kit. Es un kit altamente específico para el virus Nipah.
• Solicitud
  Diagnóstico de la contaminación del virus Nipah
• Uso
  detección de virus Nipah en suero, plasma, tejido animal infectado o secreción usando sistemas de PCR en tiempo real.
• NIV
  La infección por el virus Nipah (NiV) es una zoonosis viral causada por el virus Nipah del género Henipavirus en animales y humanos.
• Enfermedades
  encefalitis, enfermedades respiratorias.
• Host del virus
  murciélagos frugívoros de la familia Pteropodidae, que pertenece al género Pteropus.
• Brote
  India, China, Bangladesh, Tailandia …
• Contenido del kit
  NiV Super Mix, 480μl Mezcla de enzima RT-PCR, 28μl Agua de calidad molecular, 400μl Control interno, 30μl Control positivo de NiV, 30μl
• Almacenamiento
  Todos los reactivos deben almacenarse a -20 ° C. No se recomienda el almacenamiento a + 4 ° C.
• Duracion
  Todos los reactivos pueden usarse hasta la fecha de caducidad indicada en la etiqueta del kit
• Precauciones
  Descongele y congele varias veces (> 3 veces) ya que esto puede reducir la sensibilidad de la prueba. Enfríe todos los reactivos durante los pasos de trabajo. Super Mix debe almacenarse en la oscuridad.
• Instrumentos
  PE5700, MJ-Opticon y otros sistemas de un solo color y ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 y otros sistemas de varios colores
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  Virus   Nipah   NIV   Reactivo   III  
• Gene symbol
  CPLX3, MGAT3, MGST3, P4HA3, CASP5, IGHVIII-82, THAP4, MT-CSB3, IGHVIII-44, IGHVIII-67-4
• Short name
  Virus Nipah (NIV) Reactivo III y IV-RT PCR
• Technique
  RT PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. RT PCR is reverse transcription polymerase chain reaction to quantitate mRNA messenger RNA that after cDNA transcription. NiV Gen uses this as a fast way to measure gene expression.
• Species
  Virus, Viruses
• Alternative name
  Virus Nipah (NIV) Reactivo III y IV-RT PCR test kit
• Alternative technique
  dna-amplification

Gene info

• Identity
  HGNC:27652
• Gene
  CPLX3
• Long gene name
  complexin 3
• Synonyms
  • CPX-III
• GenBank acession
  • BC018026
• Locus
  15q24.1
• Discovery year
  2005-08-02
• Entrez gene record
  594855
• Pubmed identfication
  • 15911881
• RefSeq identity
  • NM_001030005
• VEGA ID
  OTTHUMG00000142816

Gene info

• Identity
  HGNC:7046
• Gene
  MGAT3
• Long gene name
  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• Synonyms gene name
  • mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• Synonyms
  • GNT-III
• GenBank acession
  • D13789
• Locus
  22q13.1
• Discovery year
  1993-08-27
• Entrez gene record
  4248
• Pubmed identfication
  • 8370666
• RefSeq identity
  • NM_002409
• Classification
  • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• VEGA ID
  OTTHUMG00000030185

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:30135
• Gene
  P4HA3
• Long gene name
  prolyl 4-hydroxylase subunit alpha 3
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
  • prolyl 4-hydroxylase, alpha polypeptide III
• Synonyms
  • C-P4Halpha(III)
• Synonyms name
  • collagen prolyl 4-hydroxylase alpha(III)
• GenBank acession
  • AY327887
• Locus
  11q13.4
• Discovery year
  2004-05-12
• Entrez gene record
  283208
• Pubmed identfication
  • 14500733
• RefSeq identity
  • NM_182904
• VEGA ID
  OTTHUMG00000165254

Gene info

• Identity
  HGNC:1506
• Gene
  CASP5
• Long gene name
  caspase 5
• Synonyms gene name
  • caspase 5, apoptosis-related cysteine protease
  • caspase 5, apoptosis-related cysteine peptidase
• Synonyms
  • ICE(rel)III
• Locus
  11q22.3
• Discovery year
  1996-09-13
• Entrez gene record
  838
• Pubmed identfication
  • 7797592
  • 9250871
• RefSeq identity
  • NM_004347
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000048073

Gene info

• Identity
  HGNC:5709
• Gene
  IGHVIII-82
• Long gene name
  immunoglobulin heavy variable (III)-82 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-82
  • immunoglobulin heavy variable (III)-82 pseudogene
• Synonyms
  • IGHV(III)-82
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28338
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151732

Gene info

• Identity
  HGNC:23187
• Gene
  THAP4
• Long gene name
  THAP domain containing 4
• Synonyms
  • CGI-36
  • Nb(III)
• Synonyms name
  • nitrobindin
• GenBank acession
  • AF258556
• Locus
  2q37.3
• Discovery year
  2003-10-08
• Entrez gene record
  51078
• Pubmed identfication
  • 12575992
  • 10810093
  • 30524950
• RefSeq identity
  • NM_015963
• Classification
  • THAP domain containing
• VEGA ID
  OTTHUMG00000133410

Gene info

• Identity
  HGNC:7426
• Gene
  MT-CSB3
• Long gene name
  mitochondrially encoded conserved sequence block III
• Synonyms gene
  • MTCSB3
• Synonyms gene name
  • conserved sequence block III
• Synonyms
  • CSB-III
• Locus
  mitochondria
• Discovery year
  1989-10-11
• Pubmed identfication
  • 2982153
• Classification
  • Mitochondrially encoded regions

Gene info

• Identity
  HGNC:5700
• Gene
  IGHVIII-44
• Long gene name
  immunoglobulin heavy variable (III)-44 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-44
  • immunoglobulin heavy variable (III)-44 pseudogene
• Synonyms
  • IGHV(III)-44
• GenBank acession
  • M99673
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28345
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152065

Gene info

• Identity
  HGNC:5707
• Gene
  IGHVIII-67-4
• Long gene name
  immunoglobulin heavy variable (III)-67-4 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-67-4
  • immunoglobulin heavy variable (III)-67-4 pseudogene
• Synonyms
  • IGHV(III)-67-4
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28340
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151874

MeSH Data

• Name
  Nucleic Acid Amplification Techniques
• Concept
  Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
• Tree numbers
  • E05.393.620
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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