Goat secondary antibody to human IgG (H+L)-Biotin
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Catalog number
MBS355656
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Price
Please ask
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Size
0.1 mL
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Products_type
Secondary Antibody
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Products_short_name
[Goat anti-human IgG]
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Products_name_syn
[Goat anti-human IgG (H+L)-Biotin conjugated]
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Reactivity
Human
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Specificity
It can specifically recognize the heavy chains on human IgG and the light chains on all human immunoglobulins. And Immunoelectrophoresis shows bands only in the gamma region against normal human serum.
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Purity
This product was purified from antiserum by immunoaffinity chromatography.
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Form
Liquid
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Storage_stability
Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
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Tested_application
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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French translation
anticorps
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Gene target
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Short name
Goat secondary antibody IgG (H+L)-
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Technique
Antibody, Goat, IgG, antibodies against human proteins, antibodies for, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
Biotin
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Species
Human, Goats, Humans
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Alternative name
caprine secondary detector Antibody (antibody to-) to H. sapiens Immunoglobulin G (heavy + light chain)-biotinilated
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Alternative technique
antibodies, caprine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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