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Synonyms
Protein A/G/L
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Alternative_names
Protein A/G/L
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Description
Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Purity
≥95%
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Binding Capacity
Under optimal conditions, 1 mg protein A/G/L will bind approximately 5-15 mg human IgG. Optimal binding of Protein A/G/L to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
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Molecular Weight
101.0 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized with no additives
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Reconstitution Instructions
Reconstitution in H₂O to a concentration of 10 mg/ml gives a clear solution.
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Background Information
Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains 6x His-tag on the N-terminus with five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody. Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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