Yeast Recombinant AHA1 Protein
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Catalog numberSPR-314C
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PricePlease ask
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Size2x100 µg
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Stock availabilityIn Stock
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Scientific contextChaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
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Protein targetAHA1
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Protein reactivityYeast
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Certificate of analysisThis product has been certified >90% pure using SDS - PAGE analysis.
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Protein descriptionYeast Recombinant AHA1 Protein
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Other nameAha1 Protein, SHSA1 Protein, HSPC322 Protein, p38 Protein
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Primary research areaCancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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CategoryProtein
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Brand namenone
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OriginRecombinant
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NCBI numberNM_001180522.1
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Gene number851800
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Protein numberQ12449
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Verified applicationsWB, SDS-PAGE, Functional Assay
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Relevant bio activityAHA1 Protein
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Protein expression modelE. coli
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Protein charastericsSee included datasheet.
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Peptide sequenceMGHHHHHHMVVNNPNNWHWVDKNCIGWAKEYFKQKLVGVEAGSVKDKKYAKIKSVSSIEGDCEVNQRKGKVISLFDLKITVLIEGHVDSKDGSALPFEGSINVPEVAFDSEASSYQFDISIFKETSELSEAKPLIRSELLPKLRQIFQQFGKDLLATHGNDIQVPESQVKSNYTRGNQKSSFTEIKDSASKPKKNALPSSTSTSAPVSSTNKVPQNGSGNSTSIYLEPTFNVPSSELYETFLDKQRILAWTRSAQFFNSGPKLETKEKFELFGGNVISELVSCEKDKKLVFHWKLKDWSAPFNSTIEMTFHESQEFHETKLQVKWTGIPVGEEDRVRANFEEYYVRSIKLTFGFGAVL
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Protein purificationAffinity Purified
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Purity pourcentage>90% High purity
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Recommended buffer for storage12.5mM Hepes buffer pH7.2, 10mM NaCl, 50% glycerol
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Protein concentrationLot/batch specific. See included datasheet.
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Protein specificity~405 kDa
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Protein tagHis tag
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Supplementary useful informationPlease see included datasheet or contact us
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Protein cell localizationCytoplasm
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Bibliography1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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Release date1-Aug-2011
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PubMed numberNot added. Please refer to PubMed
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Tested applicationsto be tested
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Tested species reactivityto be tested
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Representative figure legendSDS-PAGE of ~38kDa his-tagged mouse Aha2 protein (SPR-314). SDS-Page of yeast AHA2 Protein (SPR-314)
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WarningsNon-hazardous materials
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Protein originCanada
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Total weight kg1.4
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Net weight g0.2
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolAHSA1, AHSA2P
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Short nameYeast Recombinant AHA1 Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostYeast, Ascomycota
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SpeciesYeast, Yeasts
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Alternative nameYeast Rec. AHA1 Protein
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameactivator of HSP90 ATPase activity 1
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Synonyms gene
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Synonyms gene name
- chromosome 14 open reading frame 3
- AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-07-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactivator of HSP90 ATPase homolog 2, pseudogene
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Synonyms gene
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Synonyms gene name
- AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast)
- activator of HSP90 ATPase homolog 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-04
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data