IL-2, murine recombinant

Synonyms

T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.
Alternative_names

T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.
Description

A regulator of lymphocyte activities
Recombinant

Yes
Source

E. coli
Purity by SDS PAGE

≥98%
Assay

SDS-PAGE
Purity

≥98%
Biological activity

The ED₅₀ range is 0.1-0.4 ng/ml, as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.
Results

0.1-0.4 ng/ml
Molecular Weight

17.2 kDa
Storage Temp

-20°C
Shipping

Gel pack
Shelf Life

12 months
Appearance

Lyophilized protein
Physical form description

Lyophilized from 10 mM NaCitrate, pH 4.0
Reconstitution Instructions

Reconstitute in 100 mM AcOH to a concentration of 0.1-1 µg/µl to regain full activity. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.
Background Information

Interleukin-2 (IL-2) is a potent lymphoid cell growth factor that exerts its biological activity primarily on T cells. The recombinant Murine IL-2 is a 17.2 kDa protein containing 149 amino acid residues.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet
Additional source

Recombinants or rec. proteins
Group

recombinants

Gene target

IL-2 murine
Gene symbol

IL2, IL36G, IL25, IL22, IL18, IL37, IL1RAPL2, IL6R, IL17C, IL17D
Short name

IL-2, murine recombinant
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

Interleukin-2, murine Rec.
Alternative technique

rec

Identity

HGNC:5997
Gene

IL1RAPL2
Long gene name

interleukin 1 receptor accessory protein like 2
Synonyms
- IL-1R9
- TIGIRR-1
- IL1RAPL-2
- IL1R9
GenBank acession
- AF181285
Locus

Xq22.3
Discovery year

1999-11-19
Entrez gene record

26280
Pubmed identfication
- 10757639
RefSeq identity
- NM_017416
Classification
- TIR domain containing
- Immunoglobulin like domain containing
- Interleukin receptors
VEGA ID

OTTHUMG00000022141
Locus Specific Databases
- Global Variome shared LOVD

Identity

HGNC:6019
Gene

IL6R
Long gene name

interleukin 6 receptor
Synonyms
- CD126
- IL-6R
- IL-1Ra
- IL6RA
- gp80
Synonyms name
- membrane glycoprotein 80
- interleukin 6 receptor subunit alpha
GenBank acession
- X12830
Locus

1q21.3
Discovery year

2001-06-22
Entrez gene record

3570
Pubmed identfication
- 8428753
- 12633863
RefSeq identity
- NM_000565
Classification
- CD molecules
- Interleukin receptors
- Immunoglobulin like domain containing
VEGA ID

OTTHUMG00000036073

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data