DNA Ligase IV/LIG4 Antibody, ALEXA FLUOR 488
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Catalog number
bs-1677R-A488
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Price
Please ask
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Size
100ul
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Long name
DNA Ligase IV/LIG4 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
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Also known as
Anti-DNA Ligase IV/LIG4 PAb ALEXA FLUOR 488
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 488
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
DNA Ligase IV/LIG4
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Specificity
This is a highly specific antibody against DNA Ligase IV/LIG4.
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Modification
Unmodified
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Modification Site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
60-110/911
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Subcellular location
Nucleus
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DNA Ligase IV
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Gene ID Number
3981
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Swiss Prot
P49917
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
499nm/519nm
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Synonyms
LIG4S; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-DNA Ligase IV/LIG4 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor
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French translation
anticorps
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Gene target
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Gene symbol
LIG4, PRKDC, HLA-DOA
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Short name
Anti-DNA Ligase IV/LIG4
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Technique
Antibody, dna, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 488
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Alternative name
DNA Ligase IV/LIG4 Polyclonal Antibody, ALEXA FLUOR 488
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Alternative technique
antibodies
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Alternative to gene target
ligase IV, DNA, ATP-dependent, LIG4S, LIG4 and IDBG-49744 and ENSG00000174405 and 3981, metal ion binding, nuclei, Lig4 and IDBG-131943 and ENSMUSG00000049717 and 319583, LIG4 and IDBG-633610 and ENSBTAG00000015868 and 781252
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Gene info
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Identity
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Gene
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Long gene name
DNA ligase 4
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Synonyms gene name
- ligase IV, DNA, ATP-dependent
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1995-08-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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