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Human CellExp CD55 DAF human recombinant

Human CellExp CD55 DAF human recombinant is available 4 times from Biovision labs

7432-10 | Human CellExp CD55/DAF, human recombinant size: 10 μg | 390.98 USD

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7432-50 | Human CellExp CD55/DAF, human recombinant size: 50 μg | 882.68 USD

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7432-10 | Human CellExp™ CD55/DAF, human recombinant size: 10 μg | 291.46 USD

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7432-50 | Human CellExp™ CD55/DAF, human recombinant size: 50 μg | 825.81 USD

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Synonyms CD55, DAF, CR, CROM, TC
Alternates names CD55, DAF, CR, CROM, TC
Taglines A membrane protein that regulates the complement system on the cell surface.
Biomolecule/Target CD55/DAF
NCBI Gene ID #. 1604
NCBI Gene Symbol CD55
Gene Source Human
Accession # P08174
Recombinant Yes
Source HEK293 cells
Purity N/A
Activity (Specifications/test method) N/A
Biological activity Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 μg/ml (100 μl/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15­-2.0 μg/ml.
Results Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 μg/ml (100 μl/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15­-2.0 μg/ml.
Molecular Weight This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 35.8 kDa. The predicted N-terminus is Asp 35 . DTT-reduced Protein migrates as 50-65 kDa due to glycosylation.
Storage Temp. -20°C
Shipping Gel Pack
Shelf Life 1 year
Concentration N/A
Appearance Lyophilized
Physical form description Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Target CD55/DAF
Background CD55, also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.
Molecular weight This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 35.8 kDa. The predicted N-terminus is Asp 35 . DTT-reduced Protein migrates as 50-65 kDa due to glycosylation.
Synonym CD55, DAF, CR, CROM, TC
Other name CD55, DAF, CR, CROM, TC
NCBI gene number 1604
NCBI gene CD55
Gene source Human
NCBI number P08174
Purification ≥95%
Tested applications SDS-PAGE, N/A
Tested activity N/A
Reesults Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 μg/ml (100 μl/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15­-2.0 μg/ml.
Binding ability N/A
Unit N/A
Storage condition -20°C
Shipping under Gel Pack
Physical appearance Lyophilized
Physical properties Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence N/A
Before use Centrifuge the vial prior to opening.
Notes For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Technique Recombinant
Alternative technique rec
Alternative to gene target CD55 molecule, decay accelerating factor for complement (Cromer blood group), CR and CROM and DAF and TC, CD55 and IDBG-106331 and ENSG00000196352 and 1604, lipid binding, Extracellular, CD55 and IDBG-629269 and ENSBTAG00000006984 and 518609
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