Human CellExp™ CD55/DAF, human recombinant

  • Catalog number
    7432-50
  • Price
    Please ask
  • Size
    50 ug
  • Synonyms
    CD55, DAF, CR, CROM, TC
  • Alternative_names
    CD55, DAF, CR, CROM, TC
  • Description
    A membrane protein that regulates the complement system on the cell surface.
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 ug/ml (100 ul/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15­-2.0 ug/ml.
  • Results
    Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 ug/ml (100 ul/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15­-2.0 ug/ml.
  • Molecular Weight
    This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 35.8 kDa. The predicted N-terminus is Asp 35 . DTT-reduced Protein migrates as 50-65 kDa due to glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
  • Background Information
    CD55, also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    CD55
  • Short name
    CellExp™ CD55/DAF, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ CD55 molecule, decay accelerating factor to measure complement (Cromer blood group)/DAF, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    CD55 molecule, decay accelerating factor for complement (Cromer blood group), CR and CROM and DAF and TC, CD55 and IDBG-106331 and ENSG00000196352 and 1604, lipid binding, Extracellular, CD55 and IDBG-629269 and ENSBTAG00000006984 and 518609
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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