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Human Recombinant AKR1C1

Human Recombinant AKR1C1 is available 1 time from Biovision labs

6336-100 | Human Recombinant AKR1C1 size: 100 ug | 467.14 USD

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Synonyms Human Recombinant AKR1C1
Alternative_names Aldo-keto reductase family 1, member C 1, 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1
Description Involved in in the detoxification of aldehydes and ketones
Recombinant Yes
Source E. coli
Purity by SDS-PAGE ≥90%
Assay SDS-PAGE
Biological activity Specific activity is approximately 0.15 – 0.2 units/mg.
Unit Definition Specific activity is approximately 0.15 – 0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
Molecular Weight 38.9 kDa
Storage Temp. -20°C
Shipping gel pack
Shelf Life 12 months
Concentration 0.5 mg/ml
Appearance Liquid
Physical form description 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.
Background Information Aldo-keto reductase family 1 member C1 in humans is encoded by the AKR1C1 gene. AKR1C1 transfers progesterone to its inactive state or in other words catalyzes the reaction of 20-alpha-hydroxy progesterone (20-alpha-OHP) in the liver and intestine. AKR1C1 transfers bile and monitors the intrahepatic bile acid concentration though it has a low bile-binding ability. AKR1C1 participates in myelin formation. AKR1C1 is part of the aldo/keto reductase superfamily, which has over 40 known enzymes which catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors thus display overlapping but distinct substrate specificity.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetAKR1C1
Short name Recombinant AKR1C1
Technique Recombinant
Species Human
Alternative name H. sapiens recombinant AKR1C1
Alternative technique rec
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