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Synonyms
GREM1, CKTSF1B1, DAND2, DRM, PIG2
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Alternative_names
GREM1, CKTSF1B1, DAND2, DRM, PIG2
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Description
A TGF-β antagonist
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
> 95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Human BMP-2, at 2 ug/Ml (100 uL/well) can bind Human Gremlin, Fc Tag with a linear range of 0.8-80 ng/mL.
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Binding Capacity
Immobilized Human BMP-2, at 2 ug/Ml (100 uL/well) can bind Human Gremlin, Fc Tag with a linear range of 0.8-80 ng/mL.
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Molecular Weight
44.2 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
GREM1-AS1, GREM1
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Short name
CellExp™ Gremlin / GREM1, Fc Tag, recombinant
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Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
Fc Tag
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Species
Humans
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Alternative name
H. sapiens CellExp™ Gremlin / gremlin 1, fragment c detection labelled, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
gremlin 1, GREM1 and IDBG-5193 and ENSG00000166923 and 26585, receptor a this GO nist activity, Extracellular, Grem1 and IDBG-197200 and ENSMUSG00000074934 and 23892, GREM1 and IDBG-648566 and ENSBTAG00000046604 and 539079
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