Human SAG(S Antigen) EIA Kit

• Catalog number
  E-EL-H0940-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human SAG. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SAG. You can calculate the concentration of Human SAG in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.1ng/mL
• Detection Range
  0.16~10ng/mL
• Target Name
  SAG
• UNIProt ID
  Q9UBF6
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human SAG(S Antigen) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  Antigens are peptides or recombinant or native dependent on the production method. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  SAG   Kit  
• Gene symbol
  SAG, RNF7
• Short name
  SAG(S Antigen) EIA Kit
• Technique
  antigen, EIA, antigenes, eia
• Species
  Human, Humans
• Alternative name
  H. sapiens SAG(S protein) EIA reagent
• Alternative technique
  antigenes, kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:10521
• Gene
  SAG
• Long gene name
  S-antigen visual arrestin
• Synonyms gene name
  • S-antigen; retina and pineal gland (arrestin)
• Synonyms
  • ARRESTIN
  • RP47
• Synonyms name
  • arrestin 1
  • rod arrestin
• Locus
  2q37.1
• Discovery year
  1989-09-19
• Entrez gene record
  6295
• Pubmed identfication
  • 2249983
• RefSeq identity
  • NM_000541
• Classification
  • Classical arrestins
• VEGA ID
  OTTHUMG00000153213
• Locus Specific Databases
  • Mutations of the Arrestin Gene

Gene info

• Identity
  HGNC:10070
• Gene
  RNF7
• Long gene name
  ring finger protein 7
• Synonyms
  • SAG
  • ROC2
  • CKBBP1
• Synonyms name
  • sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
• GenBank acession
  • AF092878
• Locus
  3q23
• Discovery year
  1999-05-28
• Entrez gene record
  9616
• Pubmed identfication
  • 10082581
  • 10230407
• RefSeq identity
  • NM_014245
• Classification
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000141305

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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