Other name Biotin-6-aminohexanoic acid-N-hydroxysuccinimide ester; Biotin-X-NHS
Supplied with N/A
Description Biotin-LC-NHS is a useful reagent for biotinylation of primary amines under alkaline conditions (pH~8-9). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc. Inserts a 6-atom spacer between biotin and the target ligand, thus minimizing the steric hindrance.
Molecular formula C₂₀H₃₀N₄O₆S
Is it cell-permeable? No
Molecular weight 454.54
Storage condition -20 ̊C
Shipping condition gel pack
Purification ≥95% by HPLC
Peptide sequence N/A
Is this a salt? No
CAS number 72040-63-2
Storage instructions Protect from air and moisture
Maximum time for storage 36 months
Physical appearance White solid
Reconstitute instructions DMSO (20 mg/ml) or DMF (20 mg/ml)
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Additional description For cells, cell lines and tissues in culture till half confluency.