DDX42 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

DDX42 Lentiviral Vector CMV pLenti-GIII-CMV-RFP-2A-Puro
Gene symbol

TRIM27, IRX5, PLA2G3, IGHD2OR15-2A, EIF2A, DDX42, CXCL2, RNVU1-2A, PLPP1, CHD3
Short name

DDX42 Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
Technique

Vectors
Label

puro
Species

Human, Humans
Alternative name

DEAD (L-aspartic acid-L-gutamic acid-alanine-L-aspartic acid) box polypeptide 42 Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-Red Fluorescent-2A-Puro)
Alternative to gene target

DEAD (Asp-Glu-Ala-Asp) box polypeptide 42, DDX42P and RHELP and RNAHP and SF3b125 and SF3B8, DDX42 and IDBG-63647 and ENSG00000198231 and 11325, poly(A) RNA binding, nuclei, Ddx42 and IDBG-213407 and ENSMUSG00000020705 and 72047, DDX42 and IDBG-641673 and ENSBTAG00000021058 and 100140430
Virus

cmv

Identity

HGNC:18676
Gene

DDX42
Long gene name

DEAD-box helicase 42
Synonyms gene name
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
- DEAD (Asp-Glu-Ala-Asp) box helicase 42
Synonyms
- RNAHP
- RHELP
- SF3b125
- SF3B8
Synonyms name
- splicing factor 3b, subunit 8
GenBank acession
- BC015505
Locus

17q23.3
Discovery year

2003-06-13
Entrez gene record

11325
Pubmed identfication
- 10727850
- 16397294
RefSeq identity
- NM_007372
Classification
- DEAD-box helicases
VEGA ID

OTTHUMG00000179011

Identity

HGNC:1918
Gene

CHD3
Long gene name

chromodomain helicase DNA binding protein 3
Synonyms
- Mi-2a
- ZFH
- Mi2-ALPHA
GenBank acession
- U08379
Locus

17p13.1
Discovery year

1998-03-20
Entrez gene record

1107
Pubmed identfication
- 9326634
- 7560064
RefSeq identity
- NM_001005273
Classification
- Small nucleolar RNA protein coding host genes
- NuRD complex subunits
- PHD finger proteins
VEGA ID

OTTHUMG00000150427

Name

Artificial Gene Fusion
Concept

Scope note: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Tree numbers
- E05.393.220.250
Qualifiers

drug effects, methods, radiation effects