WT1 Antibody (Wilms Tumor protein)

• Catalog number
  F41819-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  WT1 (Wilms Tumor protein)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  IF, FACS, WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
• Notes
  Titration of the WT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This WT1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P19544
• Purity
  Antigen affinity
• Description
  Wilms Tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].
• Immunogen
  A portion of amino acids 346-375 from the human protein was used as the immunogen for this WT1 antibody.
• Storage
  Aliquot the WT1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  WT1   Wilms   Tumor   protein  
• Gene symbol
  WT1-AS, WT1
• Short name
  Anti-WT1 (Wilms Tumor protein)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to WT1 (Wilms Tumor protein)
• Alternative technique
  antibodies
• Alternative to gene target
  Wilms tumor 1, AWT1 and EWS-WT1 and GUD and NPHS4 and WAGR and WIT-2 and WT33, WT1 and IDBG-37982 and ENSG00000184937 and 7490, C2H2 zinc finger domain binding, nuclei, Wt1 and IDBG-194872 and ENSMUSG00000016458 and 22431, WT1 and IDBG-636230 and ENSBTAG00000047268 and 782183
• Tissue
  tumor

Gene info

• Identity
  HGNC:18135
• Gene
  WT1-AS
• Long gene name
  WT1 antisense RNA
• Synonyms gene
  • WIT1
• Synonyms gene name
  • Wilms tumor upstream neighbor 1
  • WT1 antisense RNA (non-protein coding)
• Synonyms
  • WIT-1
  • WT1AS
  • WT1-AS1
• Synonyms name
  • Wilms tumor associated protein
• GenBank acession
  • BC002734
• Locus
  11p13
• Discovery year
  2005-10-28
• Entrez gene record
  51352
• Pubmed identfication
  • 2173145
  • 8406502
  • 17210670
• RefSeq identity
  • NR_023920
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000039557

Gene info

• Identity
  HGNC:12796
• Gene
  WT1
• Long gene name
  WT1 transcription factor
• Synonyms gene
  • GUD
• Synonyms gene name
  • Wilms tumor 1
• Synonyms
  • WAGR
  • WIT-2
  • AWT1
  • NPHS4
  • WT-1
• Locus
  11p13
• Discovery year
  1989-04-13
• Entrez gene record
  7490
• Pubmed identfication
  • 14681303
  • 9810227
• RefSeq identity
  • NM_000378
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000039556
• Locus Specific Databases
  • LRG_525

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products