ApoBrdU-IHC DNA Fragmentation Assay Kit
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Catalog number
K403-50
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Price
Please ask
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Size
50 assays
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Description
Two-Color TUNEL Assay
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Summary
• Species reactivity- Mammalian • Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
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Sample Type
Tissue sections and fixed cell preparations
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Features Benefits
• Simple procedure; convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
ApoBrdU-IHC DNA Fragmentation Assay Kit
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Technique
Assay, dna, ihc, assays
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Host
Assay
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Alternative name
ApoBrdU-Immunohistochemistry Desoxyribonucleic acid Fragmentation test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
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Tree numbers
- E01.370.225.500.607.512
- E01.370.225.750.551.512
- E05.200.500.607.512
- E05.200.750.551.512
- E05.478.583
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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