ApoBrdU-IHC DNA Fragmentation Assay Kit

• Catalog number
  K403-50
• Price
  Please ask
• Size
  50 assays

• Description
  Two-Color TUNEL Assay
• Summary
  • Species reactivity- Mammalian • Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
• Sample Type
  Tissue sections and fixed cell preparations
• Features Benefits
  • Simple procedure; convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  ApoBrdU-IHC   DNA   Fragmentation   Kit  
• Gene symbol
  PRKDC, HLA-DOA
• Short name
  ApoBrdU-IHC DNA Fragmentation Assay Kit
• Technique
  Assay, dna, ihc, assays
• Host
  Assay
• Alternative name
  ApoBrdU-Immunohistochemistry Desoxyribonucleic acid Fragmentation test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:9413
• Gene
  PRKDC
• Long gene name
  protein kinase, DNA-activated, catalytic subunit
• Synonyms gene
  • HYRC
  • HYRC1
• Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
• Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
• Synonyms name
  • DNA-dependent protein kinase catalytic subunit
• Locus
  8q11.21
• Discovery year
  1993-11-09
• Entrez gene record
  5591
• Pubmed identfication
  • 7638222
• RefSeq identity
  • NM_001081640
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164239
• Locus Specific Databases
  • LRG_162

Gene info

• Identity
  HGNC:4936
• Gene
  HLA-DOA
• Long gene name
  major histocompatibility complex, class II, DO alpha
• Synonyms gene
  • HLA-DZA
  • HLA-DNA
• Synonyms
  • HLA-D0-alpha
• GenBank acession
  • M31525
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3111
• Pubmed identfication
  • 2370084
• RefSeq identity
  • NM_002119
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031211
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Immunohistochemistry
• Concept
  Scope note: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
• Tree numbers
  • E01.370.225.500.607.512
  • E01.370.225.750.551.512
  • E05.200.500.607.512
  • E05.200.750.551.512
  • E05.478.583
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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