PDGF-CC, human recombinant
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Catalog number7183-50
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PricePlease ask
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Size50 ug
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SynonymsPlatelet-Derived Growth Factor-CC
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Alternative_namesPlatelet-Derived Growth Factor-CC
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DescriptionPotent mitogen for a variety of cell types
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Activity Specifications test methodDetermined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.
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Biological activityDetermined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.
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Molecular Weight25 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionSterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium citrate, pH 3.0
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background InformationThe platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet α-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF-Rα receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-Rβ receptor. Recombinant human PDGF-CC is a 25kDa protein consisting of two identical disulfide-linked 112 amino-acid polypeptide chains.
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Amino acid sequenceMVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPDGFA, CCR3, CCR4, CCR2, CCR5
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Short namePDGF-CC, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namePDGF-CC, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameplatelet derived growth factor subunit A
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Synonyms gene name
- platelet-derived growth factor alpha polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 3
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Synonyms gene
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Synonyms gene name
- chemokine (C-C motif) receptor 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-05-30
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Entrez gene record
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 4
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Synonyms gene name
- chemokine (C-C motif) receptor 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-05-15
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Entrez gene record
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Pubmed identfication
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 2
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Synonyms gene
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Synonyms gene name
- chemokine (C-C motif) receptor 2
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Synonyms
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Locus
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Discovery year1995-05-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 5
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Synonyms gene
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Synonyms gene name
- chemokine (C-C motif) receptor 5
- chemokine (C-C motif) receptor 5 (gene/pseudogene)
- C-C motif chemokine receptor 5 (gene/pseudogene)
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Synonyms
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Locus
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Discovery year1996-05-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
