Sheep anti Bovine IgG H chain HRP

• Catalog number
  43R-IS001hrp
• Price
  Please ask
• Size
  1 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Raised in
  Sheep
• Specificity
  Bovine
• Cross Reactivity
  Should be determined by the end user
• Isotypes
  IgG
• Conjugate Tag
  HRP
• Method of Purification
  Sheep anti Bovine IgG (H chain) (HRP) was purified by affinity chromatography.
• Concentration
  1.0 mg/ml
• Form Buffer
  PBS with 0.1% Pro-Clean 400.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; ICC; IHC; WB
• Usage Recommendations
  ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  chain  
• Gene symbol
  NEFH, ATCAY, ATP12A
• Short name
  anti IgG H chain
• Technique
  anti, IgG, antibody to, IgGs
• Host
  Sheep
• Isotype
  IgG, IgG
• Label
  HRP
• Species
  Bovine, Bos Taurus genes are available form the Bovine Genome database. The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  Sheep anti Bovine IgG (H chain) secondary antibody (HRP)
• Alternative technique
  antibodies, immunoglobulins

Gene info

• Identity
  HGNC:7737
• Gene
  NEFH
• Long gene name
  neurofilament heavy chain
• Synonyms gene name
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament heavy polypeptide
  • neurofilament heavy
• Synonyms
  • NF-H
  • NFH
• Locus
  22q12.2
• Discovery year
  1988-08-31
• Entrez gene record
  4744
• RefSeq identity
  • NM_021076
• Classification
  • Intermediate filaments Type IV
• VEGA ID
  OTTHUMG00000151155
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Gene info

• Identity
  HGNC:779
• Gene
  ATCAY
• Long gene name
  ATCAY kinesin light chain interacting caytaxin
• Synonyms gene name
  • ataxia, cerebellar, Cayman type
  • ATCAY, caytaxin
• Synonyms
  • BNIP-H
• Synonyms name
  • Cayman ataxia
  • caytaxin
• Locus
  19p13.3
• Discovery year
  1997-01-10
• Entrez gene record
  85300
• Pubmed identfication
  • 8845847
  • 14556008
• Classification
  • BCH domain containing
• VEGA ID
  OTTHUMG00000181836

Gene info

• Identity
  HGNC:13816
• Gene
  ATP12A
• Long gene name
  ATPase H+/K+ transporting non-gastric alpha2 subunit
• Synonyms gene
  • ATP1AL1
• Synonyms gene name
  • ATPase, Na+/K+ transporting, alpha polypeptide-like 1
  • ATPase, H+/K+ transporting, nongastric, alpha polypeptide
• Synonyms
  • HK
  • H-K-ATPase
• Synonyms name
  • ATPase, Na+K+ transporting, alpha-1 polypeptide-like
  • potassium-transporting ATPase alpha chain 2
  • proton pump
  • non-gastric H(+)/K(+) ATPase alpha subunit
  • sodium/potassium ATPase, alpha polypeptide-like
• GenBank acession
  • L42558
• Locus
  13q12.12
• Discovery year
  2000-11-09
• Entrez gene record
  479
• Pubmed identfication
  • 8838794
  • 2842249
  • 25322912
• RefSeq identity
  • NM_001676
• Classification
  • ATPase H+/K+ transporting
• VEGA ID
  OTTHUMG00000016588

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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