IgG2b Isotype Control Fc fusion protein FITC

  • Catalog number
    35R-0012
  • Price
    Please ask
  • Size
    50 µg
  • Category
    Biological Reagents
  • Antibody Subtype
    Isotype Controls
  • Area of research
    Immunology
  • Raised in
    Mouse
  • Isotypes
    IgG2b kappa
  • Concentration
    500 ug/ml
  • Form Buffer
    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
  • Storage
    Store at 2-8 deg C. Do not freeze. Protect from light.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC
  • Assay Information
    It should be used at the same concentration as the experimental antibody.
  • URL
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • Group
    isotypes
  • Gene target
    IgG2b   protein  
  • Short name
    IgG2b Isotype Control Fc protein FITC
  • Technique
    Control, Isotype, FITC, FC, controls, Fluorescein, Isotypes and controls
  • Isotype
    IgG2b
  • Label
    FITC
  • Alternative name
    Mouse monoclonal IgG2b Isotype Control Fc fusion protein (FITC)
  • Alternative technique
    control, controls, fluorescine
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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