Mouse Anti-Dityrosine (DT) Monoclonal IgG1, Clone 10A6

  • Catalog number
    SMC-521D-A594
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    10A6
  • Immunogen
    Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
  • Antibody s full description
    Mouse Anti- Dityrosine Monoclonal IgG1 Antibody, Clone: 10A6: ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Dityrosine Antibody, Dityrosine (DT) Antibody, DT Antibody, DY Antibody
  • Raised in
    Mouse
  • Antibody s target
    Dityrosine
  • Primary research fields
    Cancer, Oxidative Stress, Protein Oxidation, Cell Signaling, Post Translational Modifications, Oxidation
  • Brandname
    MOLECULAR SIGNATURE®
  • Antibodies applications
    WB, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-521 was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Released date
    23-Mar-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine-BSA using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (SMC-521). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (SMC-521) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of Dityrosine-BSA using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (SMC-521). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (SMC-521) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Mouse Anti-Dityrosine Antibody [10A6] used in Western Blot (WB) on Dityrosine-BSA Conjugate (SMC-521) Mouse Anti-Dityrosine Antibody [10A6] used in Western Blot (WB) on Cervical Cancer cell line (HeLa) (SMC-521)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse Anti-Dityrosine (DT) Monoclonal IgG1, Clone 10A6
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 594
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toDityrosine (DT) monoclonal IgG1, clonality 10A6
  • Alternative technique
    murine, antibodies
  • Clone name
    10A6
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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