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Availability
Please contact us to check the availability
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Immunogen
Recombinant human Uvomorulin (Cadherin-E) aa 600-707
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Specificity
E-Cadherin 120 kDa
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Species reactivity
Human
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG2b
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Antibody s clone
SPM471
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
N/A
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Positive control
Small intestine, appendix, corneal epithelium
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Pretreatment
Pre-treatment with Unmasking Fluid H (Art. No. DE008) at 96-100 C improves the staining results remarkably. For cryostat material acetone fixation (ice cold!) is recommended.
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Tested applications
IHC(C, P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Frixen U.H., et al. (1991) E-Cadherin-mediated cell-cell adhesion prevents invasivness of human carcinoma cells. J. Cell Biol. 113 ff. 2. Umbas R. Schalken J.A., Aalders T.W., Carter B.S., Karthaus H.F.M., Schaafsma H.E., Debruyne F.M.J., and Isaacs W.B. (1992) Expression of the cellular adhesion molecule E-Cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52; 5104-5109. 3. Darai e., Scoazec J.Y., Walker-Combrouze F., Mlika-Cabanne N., Feldmann G., Madelenat P., and F. Potet (1997) Expression of cadherins in benign, borderline, and malignant ovarian epithelial tumors: a clinicopathologic study of 60 cases. Hum. Pathol. 28(8); 922-928. 4. Ruijter E., van de Kaa C., Aalders T. Ruiter D., Miller G., Debruyne F., and Schalken J. (1998) Heterogeneous expression of E-Cadherin and p53 in prostate cancer: clinical implications. BIOMED-II markers for prostate cancer study group. Mod. Pathol. 11(3); 276-281.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus