LC3B (Cleaved) Antibody

  • Catalog number
    6949-100
  • Price
    Please ask
  • Size
    100 µl
  • Antibody Target
    LC3
  • Target Alternative Name
    MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta.
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, Mouse. Predicted cross reactivity with bovine samples.
  • Immunogen Sequence
    This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.
  • Accession
    Q9GZQ8
  • Gene Id
    81631
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    Supplied in PBS with 0.09% (W/V) sodium azide.
  • Purification
    Affinity Purified Antibody
  • Application
    Immunohistochemistry, IF
  • Positive Control
    ICC: U251 cells, IF: SY5Y cells
  • Application And Usages
    IF: 1:10-50, ICC: 1:10-50.
  • Description
    Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    LC3B   Cleaved  
  • Short name
    LC3B (Cleaved) Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit Ig
  • Alternative name
    LC3B (Cleaved) (antibody to-)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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