PIM1(Tyr309) Antibody

• Catalog number
  bs-3353R
• Price
  Please ask
• Size
  0.1ml

• Long name
  PIM1(Tyr309) Primary Polyclonal Antibody
• Also known as
  PIM1(Tyr309) PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  PIM1 Tyr309
• Specificity
  This is a highly specific antibody against PIM1 Tyr309. This phosphorylation site is also referred to as Tyr218, as this is the position in Isoform 2 of this protein. This phosphorylation site is homologous to Tyr302 in Mouse and Tyr218 in Rat.
• Modification s
  Phosphorylation
• Modification site s
  Tyr309
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  301-328/404
• Subcellular location
  Helical
• Source
  KLH conjugated synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of Tyr218
• Gene ID number
  5292
• Swiss Prot
  P11309
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  PIM; Serine/threonine-protein kinase pim-1; PIM1
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  PIM1   Tyr309  
• Gene symbol
  PIM1, LONP1
• Short name
  Anti-PIM1 Tyr309
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  PIM1(Tyr309) Polyclonal Antibody
• Alternative technique
  antibodies
• Alternative to gene target
  pim-1 oncogene, PIM, PIM1 and IDBG-85488 and ENSG00000137193 and 5292, transferase activity, nuclei, Pim1 and IDBG-160725 and ENSMUSG00000024014 and 18712, PIM1 and IDBG-630304 and ENSBTAG00000000396 and 281402

Gene info

• Identity
  HGNC:8986
• Gene
  PIM1
• Long gene name
  Pim-1 proto-oncogene, serine/threonine kinase
• Synonyms gene
  • PIM
• Synonyms gene name
  • pim-1 oncogene
• Locus
  6p21.2
• Discovery year
  1986-01-01
• Entrez gene record
  5292
• VEGA ID
  OTTHUMG00000016426

Gene info

• Identity
  HGNC:9479
• Gene
  LONP1
• Long gene name
  lon peptidase 1, mitochondrial
• Synonyms gene
  • PRSS15
• Synonyms gene name
  • protease, serine, 15
• Synonyms
  • LonHS
  • hLON
  • PIM1
• GenBank acession
  • U02389
• Locus
  19p13.2
• Discovery year
  1999-09-17
• Entrez gene record
  9361
• Pubmed identfication
  • 8248235
  • 8119403
• RefSeq identity
  • NM_004793
• Classification
  • Serine proteases
  • AAA ATPases
• VEGA ID
  OTTHUMG00000180504

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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