Human Enhanced green fluorescence EGFP-mCherry
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Catalog number
AP50007HU
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Price
Please ask
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Size
10µg
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Long name
Recombinant Human Enhanced green fluorescence protein EGFP-mCherry
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Gene name
Enhanced green fluorescence EGFP-mCherry
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Category
Recombinant proteins
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Storage and handling
For short term storage the vial may be kept refrigerated in the temperature range of 1 to 6 degrees Celcius. For long term storage, keep the protein frozen at -20 deg. C. Avoid cycles of freezing and twaing as they may denaturate the peptide chains of the protein, thus changing its structure and properties.
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Conjugate
The Green Fluorescent Protein (eGFP) of Recombinant Enhanced green fluorescence EGFP-mCherry can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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