Alternative Names
JFY1; BBC3; BCL2 Binding Component 3
Assay Type
Sandwich
Sensitivity
0.059ng/mL
Standard
10ng/mL
Detection range
0.156-10ng/mL
Assay length
3h
Research Area
Apoptosis;Tumor immunity;
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p53 Upregulated Modulator Of Apoptosis (PUMA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to p53 Upregulated Modulator Of Apoptosis (PUMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain p53 Upregulated Modulator Of Apoptosis (PUMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of p53 Upregulated Modulator Of Apoptosis (PUMA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Description
This 1 is suited for programmed cell-death studies.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetPUMA(p53
Upregulated
Modulator
Short name
PUMA(p53 Upregulated Modulator Of Apoptosis) ELISA Kit
Technique
apoptosis, ELISA kit, ELISA
Host
antigen assay ELISA kit 1 Kit (96 Wells)
Species
Human
Alternative name
H. sapiens PUMA(p53 Upregulated Modulator on programmed cellular destruction) Enzyme-linked immunosorbent assay reagent
Alternative technique
celldeath
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
