Background information:
Granzyme B is a 27 kDa serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide corresponding to residues surrounding amino acid 221 of human Granzyme B
Concentration of antibody:
0.5 mg/ml
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps
Identity 4709
Gene GZMB
Long gene name granzyme B
Synonyms gene name- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Synonyms- CCPI
- CGL-1
- CSP-B
- CGL1
- CTSGL1
- HLP
- SECT
Synonyms name- fragmentin 2
- cytotoxic serine protease B
- cathepsin G-like 1
- T-cell serine protease 1-3E
- granzyme 2
- cytotoxic T-lymphocyte-associated serine esterase 1
Locus 14q12
Discovery year 1989-05-12
Entrez gene record 3002
Classification- Granule associated serine proteases of immune defence
Havana BLAST/BLAT OTTHUMG00000029369
