HEPES Buffer Solution (1 M)

Storage

+2°C to +8°C
Product Group

Buffer & Salt Solutions
Shelf Life

2 years
Description

Buffering solutions are useful to keep the pH range sable when using this reagent of Capricorn. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
Buffer

25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

Identity

HGNC:26506
Gene

PPM1M
Long gene name

protein phosphatase, Mg2+/Mn2+ dependent 1M
Synonyms gene name
- protein phosphatase 1M (PP2C domain containing)
- protein phosphatase, Mg2+/Mn2+ dependent, 1M
Synonyms
- PP2Ceta
- FLJ32332
Synonyms name
- protein phosphatase 2C eta
GenBank acession
- AK096681
Locus

3p21.2
Discovery year

2005-04-25
Entrez gene record

132160
Pubmed identfication
- 12477932
RefSeq identity
- NM_144641
Classification
- Protein phosphatases, Mg2+/Mn2+ dependent
VEGA ID

OTTHUMG00000153046