Rabbit Anti-Human ERK5 (pThr219 + pTyr221) Polyclonal: ATTO 680

  • Catalog number
    SPC-977D-A680
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A phospho-specific peptide corresponding to residues surrounding Thr219 and Tyr221 of human ERK2 (AA 216-222)
  • Antibody s target
    Human ERK5 (pThr219 + pTyr221)
  • Antibody s full description
    Rabbit Anti-Human ERK5 (pThr219 + pTyr221) Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Big MAP kinase 1 Antibody, BMK 1 kinase Antibody, ERK5 Antibody, BMK1 Antibody, EC 2.7.11.24 Antibody, ERK4 Antibody, ERK5 Antibody, Extracellular signal regulated kinase 5 Antibody, MAP kinase 7 Antibody, MAPK7 Antibody , Mitogen activated protein kinase 7 Antibody, MK07_HUMAN Antibody, OTTHUMP00000065906 Antibody, OTTHUMP00000065907 Antibody, PRKM7 Antibody
  • Verified applications
    WB, AM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:250); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.025% Thimerosal
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects 88.637 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:250 dilution of SPC-977 was sufficient for detection of ERK5 (pThr219 + pTyr221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Nucleus
  • Tissue specificity
    Highly expressed in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver
  • Scientific context
    ERK5 or MAPK7 is a protein-serine/threonine kinase. ERK5 is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). Upregulation of this gene has been found in breast, prostate, and liver cancer.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_002740.2
  • Gene number
    5598
  • Protein number
    Q13164
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    ERK5   pThr219   pTyr221   ATTO   680  
  • Gene symbol
    MAPK7
  • Short name
    Rabbit Anti- ERK5 (pThr219 + pTyr221) Polyclonal: ATTO 680
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    ATTO 680
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman ERK5 (pThr219 + pTyr221) polyclonal: ATTO 680
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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