BLM Antibody
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Catalog number3769-100
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PricePlease ask
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Size100 µl
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Antibody TargetBLM
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Target Alternative NameBS-Bloom Syndrome, DNA Helicase, RECQ-Like, Type 2- RECQ2, RECQL2, RECQL3
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceSynthetic peptide of human BLM
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AccessionNP_000048.1
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Gene Id641
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation
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Application And UsagesWestern blotting (1:300 – 1:3000) and Immunoprecipitation. 293T cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the BLM of human origin. Reactivity to other species has not been tested.
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DescriptionBloom’s syndrome is an autosomal recessive disorder characterized by pre- and post-natal growth deficiencies, sun sensitivity, immunodeficiency and a predisposition to various cancers. The gene responsible for Bloom’s syndrome, BLM, encodes a protein homologous to the RecQ helicase of E. coli and is mutated in most Bloom’s syndrome patients. One characteristic of Bloom’s syndrome is an increased frequency of sister chromatid exchange (SCE). BLM has been shown to unwind G4 DNA, and a failure of this function is thought to be responsible for the increased rate of SCE. BLM is known to be translocated to the nucleus, where its ATPase activity is stimulated by both single- and double-stranded DNA. Mutations in the yeast SGS1, a homolog of BLM, are known to cause mitotic hyperrecombination similiar to that observed in Bloom’s cells.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBLM
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Short nameBLM Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameBloom syndrome, RecQ helicase-like (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetBloom syndrome, RecQ helicase-like, BS and RECQ2 and RECQL2 and RECQL3, BLM and IDBG-30365 and ENSG00000197299 and 641, hydrolase activity, nuclei, Blm and IDBG-195418 and ENSMUSG00000030528 and 12144, BLM and IDBG-629490 and ENSBTAG00000020301 and 534148
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Gene info
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Identity
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Gene
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Long gene nameBLM RecQ like helicase
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Synonyms gene name
- Bloom syndrome
- Bloom syndrome RecQ like helicase
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-06
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Entrez gene record
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Pubmed identfication
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Classification
- RecQ like helicases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data