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Gene info
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
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Gene names
SMAD2, JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by protein A chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD2.
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Immuno application
ELISA, Western Blot
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Antibody advice
SMAD2 antibody can be used for detection of SMAD2 by ELISA at 1:1562500. SMAD2 antibody can be used for detection of SMAD2 by western blot at 2.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD2 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human, Mouse, Rat, Dog, Zebrafish
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Omim nr
5174511
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Ncbi nr
NP_005892
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KDa
51 kDa, 52 kDa, 48 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
4087
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GENE symbol
SMAD2
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Antigene name
SMAD family member 2
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Source
Homo sapiens
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Protein nr
Q15796
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
