-
Verified reactivity
Oryctolagus cuniculus (Rabbit)
-
Detection limits
31.25-2000 ng/mL
-
Sensitivity limit
1,02 ng/mL
-
Samples to be analyzed
Serum, plasma and other biological fluids
-
Assay duration
2 hours, 40min
-
Verified applications
CLIA
-
Assay class
Sandwich
-
ELISA detection
Chemiluminescent
-
Protein number
P53614
-
Alternate protein number
Please refer to SwissProt
-
Gene number
100009168
-
Gene name
Serum Amyloid A
-
Alternate gene name
SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
-
Estimated production time
7-11 business days
-
Shipping requirements
Blue ice
-
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
Use for
8 months
-
ELISA s specificity
This assay has high sensitivity and excellent specificity for detection of Serum Amyloid A (SAA). No significant cross-reactivity or interference between Serum Amyloid A (SAA) and analogues was observed.
-
ELISA s cross reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
-
Assay principle
The microplate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid A (SAA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serum Amyloid A (SAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serum Amyloid A (SAA) level in the sample or standard.;
-
Protocol
Please see ELISA's datasheet, otherwise contact us
-
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
-
ELISA s stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
-
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
-
Research main area
Infection immunity;Kidney biomarker;Hepatology;Urology;
-
Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
Notes
For research use only. Not for diagnostic procedures.
-
-
Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
-
About
Rabbits are used for polyclonal antibody production by bioma. Rabbit antibodies are very stable and can be stored for several days at room temperature. bioma adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
-
Latin name
Oryctolagus cuniculus
-
Group
Sera
