anti-Galectin-3 Antibody

• Catalog number
  38-170
• Price
  Please ask
• Size
  0.1 mg

• Gene info
  Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells.
• Gene names
  L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin-3, 35 kDa lectin, Gal-3
• Antibodie s label
  unlabelled serum
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized Galectin-3 matrix.
• Immuno application
  ELISA, Western Blot, immuno-histo-chemistry
• Antibody advice
  Sandwich ELISA:To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3. Western Blot:To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry:This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4 grades C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
• Keep at
  Galectin-3 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
• Works with
  Human
• Omim nr
  215274262
• Ncbi nr
  P17931
• Vial with antibody
  Lyophilized
• NCBI GENE nr
  3958
• GENE symbol
  LGALS3
• Antigene name
  lectin, galactoside-binding, soluble, 3
• Source
  Homo sapiens
• Protein nr
  P17931
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Galectin-3  
• Gene symbol
  LGALS3, LGALS3BP
• Short name
  anti-Galectin-3 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toGalectin-3 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6563
• Gene
  LGALS3
• Long gene name
  galectin 3
• Synonyms gene
  • LGALS2
• Synonyms gene name
  • lectin, galactoside-binding, soluble, 3
• Synonyms
  • MAC-2
  • GALIG
• Synonyms name
  • advanced glycation end-product receptor 3
• GenBank acession
  • M64303
• Locus
  14q22.3
• Discovery year
  1991-09-13
• Entrez gene record
  3958
• Pubmed identfication
  • 2009535
  • 8063692
• RefSeq identity
  • NM_002306
• Classification
  • Receptor ligands
  • Galectins
• VEGA ID
  OTTHUMG00000171030

Gene info

• Identity
  HGNC:6564
• Gene
  LGALS3BP
• Long gene name
  galectin 3 binding protein
• Synonyms gene name
  • lectin, galactoside-binding, soluble, 3 binding protein
• Synonyms
  • MAC-2-BP
  • 90K
  • BTBD17B
  • TANGO10B
  • M2BP
  • gp90
  • CyCAP
• Synonyms name
  • L3 antigen
  • Mac-2-binding protein
  • serum protein 90K
  • transport and golgi organization 10 homolog B (Drosophila)
• GenBank acession
  • L13210
• Locus
  17q25
• Discovery year
  1994-09-30
• Entrez gene record
  3959
• Pubmed identfication
  • 7698018
  • 8034587
  • 8390986
• RefSeq identity
  • NM_005567
• Classification
  • BTB domain containing
  • Scavenger receptor cysteine rich domain containing
  • Receptor ligands
• VEGA ID
  OTTHUMG00000177572

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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