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Available ordering format
Liquid
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Immunogen
Purified human IgG
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Shelf life is one year from date of receipt.
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Verified applications
ELISA (ELISA)
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Connected products
other secondary antibodies to human immunoglobulins
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Recommended dilutions for use
needs to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
human IgG (H&L),F(ab)'2 fragment
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Possible reactivity
human IgG (H&L),F(ab)'2 fragment
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
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References
to be added when available
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Scientific context
Goat anti-human IgG (H&L),F(ab)'2 fragment is a secondary antibody which binds to human IgG (H&L),F(ab)'2 fragment in immunological assays.
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Notes
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify Goat, , Unconjugated, min. cross-reactivity /mouse/rabbit serum by agisera by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Group
Sera
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Conjugation
not conjugated
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Gene target
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Short name
Goat, , Unconjugated, min. cross-reactivity /mouse/rabbit serum
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Technique
affinity, purified, Rabbit, Mouse, serum, Goat, goats, mouses, purifications
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Label
Unconjugated
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Species
Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Mouses
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Alternative name
caprine, protein enriched, nonconjugated, min. cross-This (antibody to-) recognizes to bovine/mouse/production species: rabbit blood serum
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Alternative technique
pure, rabbit-anti, sera, murine, caprine
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Tissue
serum
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