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Available ordering format
Liquid
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Immunogen
Purified rabbit IgG (H&L)
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
2-8°C.
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Verified applications
Contact Gentaur support center
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Connected products
other unconjugated secondary antibodies against rabbit IgG (H&L)
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Recommended dilutions for use
This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Goat anti-rabbit IgG (H&L) is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
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Notes
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 14.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify Goat, , Unconjugated, min. cross-reactivity , mouse, serum by agisera by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Latin name
Capra aegagrus hircus, Mus musculus, Rattus norvegicus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats. Serum should be stored at +5°C
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Group
Sera
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Conjugation
not conjugated
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Gene target
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Short name
Goat, , Unconjugated, min. cross-reactivity , mouse, serum
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Technique
affinity, purified, Mouse, serum, Goat, goats, mouses, purifications
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
Unconjugated
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Species
Rat, Goats, Humans, Mouses, Rats
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Alternative name
caprine, protein enriched, nonconjugated, min. cross-This (antibody to-) recognizes to H. sapiens, mouse, rat blood serum
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Alternative technique
pure, sera, murine, caprine
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Tissue
serum
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