Human Recombinant ALDH2

• Catalog number
  6332-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant ALDH2
• Alternative_names
  Aldehyde dehydrogenase - mitochondrial, ALDH-E2, ALDHI, ALDM.
• Description
  A polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein (determined by LAL method).
• Biological activity
  Specific activity is > 0.14 units/ml
• Unit Definition
  One unit will oxidize 1.0 µmole of acetaldehyde to acetic acid per minute at pH 8.0 at 25°C in the presence of beta-NAD, potassium and thiols.
• Molecular Weight
  54.5 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 1 mM EDTA and 10% glycerol.
• Background Information
  ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. ALDH2 has been shown to interact with GroEL.
• Amino acid sequence
  MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ALDH2  
• Gene symbol
  ALDH2
• Short name
  Recombinant ALDH2
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. aldehyde dehydrogenase 2 family (mitochondrial)
• Alternative technique
  rec
• Alternative to gene target
  aldehyde dehydrogenase 2 family (mitochondrial), ALDH-E2 and ALDHI and ALDM, ALDH2 and IDBG-57710 and ENSG00000111275 and 217, oxidoreductase activity, multiple

Gene info

• Identity
  HGNC:404
• Gene
  ALDH2
• Long gene name
  aldehyde dehydrogenase 2 family member
• Synonyms gene name
  • aldehyde dehydrogenase 2 family (mitochondrial)
• GenBank acession
  • M26760
• Locus
  12q24.12
• Discovery year
  1986-01-01
• Entrez gene record
  217
• Pubmed identfication
  • 4015823
  • 2987944
• RefSeq identity
  • NM_000690
• Classification
  • Aldehyde dehydrogenases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000169603
• Locus Specific Databases
  • Human Polymorphisms of ALDH Genes

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products