Glutathione Reductase Activity Colorimetric Assay Kit

Description

Highly Sensitive Assay, HTS
Summary

• Detection method- Absorbance (405 nm) • Species reactivity- Mammalian • Application- The assay can detect 0.1 – 40 mU/ml GR in various samples.
Sample Type

Cell and tissue culture supernatants, urine, plasma and serum, erythrocyte lysates, as well as many other biological fluids, etc.
Features Benefits

• Simple procedure; takes ~ 30-40 minutes • Fast and convenient
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples.
Data Sheet Link
Test

Biovision supplies other types of Assays as 1.
Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Gene target

Glutathione Reductase Activity Kit
Short name

Glutathione Reductase Activity Colorimetric Assay Kit
Technique

colorimetric, Assay, assays, colorimetry
Host

Assay
Alternative name

GSH Reductase Activity colorimetry test reagent
Alternative technique

assays, arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832