GFP antibody biotin
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Catalog number60R-GG001bt
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PricePlease ask
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Size1 mg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Polyclonal Antibodies
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Area of researchMiscellaneous
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Type of ImmunogenGFP antibody (biotin) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria ..
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Raised inGoat
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SpecificityJellyfish GFP
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Cross ReactivityShould be determined by the end user
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Conjugate TagBiotin
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Method of PurificationGFP antibody (biotin) was purified by affinity chromatography.
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Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
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StorageStore at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; IHC; WB
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Usage RecommendationsELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Gene target
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Short nameGFP antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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Labelgfp
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Alternative nameGoat polyclonal GFP antibody (biotin) conjugated
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data