Mouse Monoclonal IgG1 Anti-Hep Par-1 (Hepatocyte Specific Antigen) [OCH1E5]

  • Catalog number
    HEP001
  • Price
    Please ask
  • Size
    1 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Formalin-fixed, rejected liver transplant
  • Specificity
    Hepatocellular antigen of the mitochondrial fraction
  • Species reactivity
    Human
  • Product presentation
    Purified mouse IgG1 in PBS containing BSA, gelatine, and sodium azide (0.09%)
  • Product category
    Primary Antibody
  • Antibody s raised in
    Mouse
  • Antibody s isotype
    IgG1
  • Antibody s clone
    OCH1E5
  • Product is supplied as
    liquid concentrate
  • Recommended concentration for use
    1:20-1:100
  • Protein concentration
    50 µg/ml
  • Positive control
    Liver
  • Pretreatment
    Formaldehyde-fixed tissue should be pre-treated with Unmasking Fluid E (TRIS/EDTA pH. 9; Art. No. DE006), citrate buffer doesn't perform equally well.
  • Tested applications
    IHC(C, P)
  • Recommended secondary reagent
    Anti-mouse IgG polymere system (e.g. VECTOR ImmPress Kit Art. No. MP-7402) or DAKO EnVISION system. For liver and kidney staining it is recommended not to use Biotin conjugates since endogenous Biotin may interfere.
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    1. Zhen Fan, Matt van de Rijn, Kelli Montgomery and Robert V Rouse. Hep Par 1 Antibody Stain for the Differential Diagnosis of Hepatocellular Carcinoma: 676 Tumours Tested Using Tissue Microarrays and Conventional Tissue Sections Mod Pathol 2003;16(2):137–144. 2. T P Thamboo, A Wee Hep Par 1 expression in carcinoma of the cervix: implications for diagnosis and prognosis Journal of Clinical Pathology 2004;57:48-53. 3. Kanitakis J, Chouvet B, Claudy A, Scoazec JY. Immunoreactivity of hepatocyte paraffin 1 monoclonal antibody in cutaneous metastatic tumors. Am J Clin Pathol. 2004 Jul;122(1):85-89.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Research
  • Technique
    Mouse monoclonal, antigen, anti-, Mouse, anti, antibody to, antibodies, antigenes, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG1
  • Species
    Mouse, Mouses
  • Alternative name
    Hep Par-1 (Hepatocyte Specific Antigen)
  • Alternative technique
    antigenes, murine, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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