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Availability
Please contact us to check the availability
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Immunogen
Formalin-fixed, rejected liver transplant
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Specificity
Hepatocellular antigen of the mitochondrial fraction
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Species reactivity
Human
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Product presentation
Purified mouse IgG1 in PBS containing BSA, gelatine, and sodium azide (0.09%)
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
OCH1E5
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:20-1:100
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Protein concentration
50 µg/ml
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Positive control
Liver
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Pretreatment
Formaldehyde-fixed tissue should be pre-treated with Unmasking Fluid E (TRIS/EDTA pH. 9; Art. No. DE006), citrate buffer doesn't perform equally well.
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Tested applications
IHC(C, P)
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Recommended secondary reagent
Anti-mouse IgG polymere system (e.g. VECTOR ImmPress Kit Art. No. MP-7402) or DAKO EnVISION system. For liver and kidney staining it is recommended not to use Biotin conjugates since endogenous Biotin may interfere.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Zhen Fan, Matt van de Rijn, Kelli Montgomery and Robert V Rouse. Hep Par 1 Antibody Stain for the Differential Diagnosis of Hepatocellular Carcinoma: 676 Tumours Tested Using Tissue Microarrays and Conventional Tissue Sections Mod Pathol 2003;16(2):137–144. 2. T P Thamboo, A Wee Hep Par 1 expression in carcinoma of the cervix: implications for diagnosis and prognosis Journal of Clinical Pathology 2004;57:48-53. 3. Kanitakis J, Chouvet B, Claudy A, Scoazec JY. Immunoreactivity of hepatocyte paraffin 1 monoclonal antibody in cutaneous metastatic tumors. Am J Clin Pathol. 2004 Jul;122(1):85-89.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus