Mouse anti Caspase-8

• Catalog number
  X1176M
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains which are removed to activate the enzyme along with cleavage into the two subunits. These subunits then for the active protease which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.
• Antibody come from
  Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-8 protein and mouse myeloma cells.
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
• Clone
  FLICE 3
• Antigen antibody binding interaction
  Mouse anti Caspase-8 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot
• Antibody s suited for
  Detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16_x000B__x000B_2. Martin, D.A., et al. Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHalpha1) death signal. J. Biol. Chem. 1998, 273, 4345-4349_x000B__x000B_3. Green, D.R., Apoptotic pathways: the roads to ruin. Cell 1998, 94, 695-698_x000B__x000B_4. Hu, S., et al. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J. Biol. Chem. 1997, 272, 17255-17257
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Caspase-8  
• Gene symbol
  CASP8, CARD8, CASP8AP2
• Short name
  Mouse anti Caspase-8
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to caspase-8
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:1509
• Gene
  CASP8
• Long gene name
  caspase 8
• Synonyms gene name
  • caspase 8, apoptosis-related cysteine protease
  • caspase 8, apoptosis-related cysteine peptidase
• Synonyms
  • MCH5
  • MACH
  • FLICE
  • Casp-8
• GenBank acession
  • U60520
• Locus
  2q33.1
• Discovery year
  1996-11-11
• Entrez gene record
  841
• Pubmed identfication
  • 8681376
  • 8681377
• RefSeq identity
  • NM_001228
• Classification
  • Caspases
  • Death effector domain containing
  • Death inducing signaling complex
  • Ripoptosome
• VEGA ID
  OTTHUMG00000132821
• Locus Specific Databases
  • CASP8base: Mutation registry for Caspase 8 deficiency
  • LRG_34

Gene info

• Identity
  HGNC:17057
• Gene
  CARD8
• Long gene name
  caspase recruitment domain family member 8
• Synonyms gene name
  • caspase recruitment domain family, member 8
• Synonyms
  • TUCAN
  • KIAA0955
  • CARDINAL
  • NDPP
  • Dakar
• GenBank acession
  • AB023172
• Locus
  19q13.33
• Discovery year
  2003-12-03
• Entrez gene record
  22900
• Pubmed identfication
  • 10231032
  • 11408476
• RefSeq identity
  • NM_014959
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000165047

Gene info

• Identity
  HGNC:1510
• Gene
  CASP8AP2
• Long gene name
  caspase 8 associated protein 2
• Synonyms gene name
  • CASP8 associated protein 2
• Synonyms
  • FLASH
  • CED-4
  • RIP25
  • FLJ11208
  • KIAA1315
• Synonyms name
  • FLICE-associated huge protein
• GenBank acession
  • AB037736
• Locus
  6q15
• Discovery year
  1999-12-10
• Entrez gene record
  9994
• Pubmed identfication
  • 10235259
  • 17245429
  • 17003126
• RefSeq identity
  • NM_001137667
• VEGA ID
  OTTHUMG00000015212

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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