Glyoxalase II (Glx II), Human Recombinant

• Catalog number
  P1072-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Hydroxyacylglutathione hydrolase, mitochondrial, HAGH1, GLO2
• Alternative_names
  Hydroxyacylglutathione hydrolase, mitochondrial, HAGH1, GLO2
• Description
  Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  ≥ 10 U/mg , in which one unit is the amount of enzyme that will hydrolyze 1.0 µmole of SLG per minute at pH 7.4 at 25 ̊C.
• Results
  ≥ 10 U/mg
• Unit Definition
  One unit is the amount of enzyme that will hydrolyze 1.0 µmole of SLG per minute at pH 7.4 at 25 ̊C.
• Molecular Weight
  32.4 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  30% glycerol, 50 mM Tris pH 7.4 and 150 mM NaCl
• Background Information
  The glyoxalase system consists of Glyoxalase I (Glx I) and Glyoxalase II (Glx II) which is responsible for detoxifying α-ketoaldehydes such as the potent and cytotoxic methylglyoxal (MG). Glx I converts MG to S-D-lactoyl-glutathione (SLG). SLG is then hydrolyzed to D-lactate and reduced glutathione by Glx II. Glx II desensitizes cells to p53-dependent DNA damage-induced apoptosis. It also plays a role during development and in the pathogenesis of cancer and neurodegenerative diseases. Glyoxalase II may also be important in the regulation of spermatogenesis.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Glyoxalase   Glx  
• Short name
  Glyoxalase II (Glx II), Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Glyoxalase II (Glx II), H. sapiens Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products