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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
chromatin immunoprecipiation (ChIP), Dot Blot (Dot), ELISA (ELISA), immunofluorescence (IF), western blot (WB)
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Connected products
AS16 3635-10 | anti-H3S10p | Histone H3 (p Ser10), rabbit antibodies, 10 µg AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
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Recommended dilutions for use
2 µg/million cells (ChIP), 1: 20 000 (Dot), 1: 2000 (IF), 1: 100 (WB)
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Molecular weight expected аpparent
15 | 17 kDa
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Verified reactivity
human
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Possible reactivity
chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in March 2016.
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Scientific context
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Phosphorylation of H3S10 is associated with mitosis.
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Notes
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0.05% sodium azide and 0.05% ProClin300.
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Protein number
Q71DI3
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify H3S10p, Histone H3 (p Ser10) ( ) by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
