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Resistin human

Resistin human is available 0 time from Biovision labs

Synonyms Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-α-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.
Alternates names Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-α-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.
Taglines A cysteine-rich protein resulting in high LDL
Biomolecule/Target Resistin
NCBI Gene ID #. 56729
NCBI Gene Symbol RETN
Gene Source Human
Accession # Q9HD89
Recombinant Yes
Source E. coli
Purity ≥98%
Activity (Specifications/test method) N/A
Biological activity N/A
Results N/A
Molecular Weight 19.5 kDa
Storage Temp. -20°C
Shipping Gel pack
Shelf Life 1 year
Concentration N/A
Appearance Lyophilized protein
Physical form description Lyophilized without additives
Reconstitution Instructions Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Kit's description BioVision’s Resistin [XCP-1 (CEBPE regulated myeloid-specific secreted cysteine-rich protein precursor 1), FIZZ3 (found in inflammatory zone 3), or ADSF (adipocyte-specific secretory factor)] Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Resistin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti-Resistin antibody, both biotinylated Resistin peptide and peptide standard or targeted peptide in samples interacts competitively with the Resistin antibody. Uncompeted (bound) biotinylated Resistin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Resistin peptide in the standard or samples. This is due to the competitive binding to Resistin antibody between biotinylated Resistin peptide and peptides in standard or samples. A standard curve of known concentration of Resistin peptide can be established and the concentration of Resistin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Resistin is 446 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV
Highlights • Detection method: Absorbance (450 nm) • Species reactivity: human, mouse, rat • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits • Easy, convenient and time-saving method to assay Resistin. • The minimum detectable concentration of Resistin is less than 446 pg/ml. • This EIA kit detects Resistin (90 aa). No other active isoforms have been reported. This kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Category Resistin
Kit's other name XCP-1 (human/mouse/rat) EIA Kit, FIZZ3 (human/mouse/rat) EIA Kit , ADSF (human/mouse/rat) EIA Kit
Contents • Resistin Microplate (Item A) coated with secondary antibody, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standard Resistin Peptide (Item C) (10 μl/vial) • Anti-Resistin polyclonal antibody (item N) (5 μl/vial) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium or other sample) diluent. (5x concentrated) • Biotinylated Resistin peptide (Item F), (20 μl/vial) • HRP-Streptavidin Concentrate (Item G), 160x concentrated • Positive Control (Item M) (100 μl) • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid
Storage condition -20°C
Shipping condition gel pack
Maximum time can be stored 6 months
Species reactivity human, mouse, rat
Samples tested • Serum & plasma • Culture supernatants & cell lysates
Storage Conditions +4°C
Tag Line NA
Shipping Conditions gel pack
Shelf life 12 months
Species Reactivity NA
Sample Type Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
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