-
Synonyms
PWTSR, THSD2, Roof plate-specific spondin-3, RSPO3
-
Alternative_names
PWTSR, THSD2, Roof plate-specific spondin-3, RSPO3
-
Description
Regulates the proliferation and differentiation of certain stem cell populations
-
Recombinant
Yes
-
Source
CHO Cells
-
Purity by SDS PAGE
≥95%
-
Assay
SDS-PAGE
-
Endotoxin Level
< 0.1 ng/ug of protein (
-
Activity Specifications test method
R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
-
Biological activity
R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
-
Molecular Weight
37.0 kDa
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized powder
-
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.
-
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
-
Background Information
The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues. Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.
-
Amino acid sequence
MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
